The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes. It was shown that using this biosensor it is possible to reach the sensitivity of ~10(-15) M. This sensitivity was comparable with that of the NW biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate promising approaches used to form the sensor elements for high-sensitive disease diagnostics.
Odnim iz bystrodeĭstvuiushchikh i vysokochuvstvitel'nykh metodov, pozvoliaiushchikh vyiavliat' potentsial'no vazhnye belkovye molekuly, iavliaetsia metod nanoprovolochnoĭ (NP) detektsii. Nanoprovolochnyĭ biosensor na osnove struktur kremniĭ na izoliatore (KNI), byl ispol'zovan dlia biospetsificheskoĭ detektsii NFAT 1 (D-NFAT 1)-onkomarkera v real'nom vremeni bez metok. Dlia étogo KNI-nanoprovoloki byli modifitsirovany aptamerami k NFAT 1, ispol'zuemymi v kachestve makromolekuliarnykh zondov. Pokazano, chto takoĭ biosensor pozvoliaet dostigat' chuvstvitel'nost' poriadka 10-15 M. Éta chuvstvitel'nost' sopostavima s chuvstvitel'nost'iu, poluchennoĭ na nanoprovolochnom biosensore s immobilizovannymi antitelami, ispol'zuemymi v kachestve makromolekuliarnykh zondov. Rezul'taty demonstriruiut perspektivnost' ispol'zovannykh v rabote podkhodov formirovaniia sensornykh élementov dlia vysokochuvstvitel'noĭ diagnostiki zabolevaniĭ.
Keywords: D-NFAT 1; aptamer; biosensor; nanowires; silicon-on-insulator.