Fulminant adenovirus hepatitis after hematopoietic stem cell transplant: Retrospective real-time PCR analysis for adenovirus DNA in two cases

J Infect Chemother. 2015 Dec;21(12):857-63. doi: 10.1016/j.jiac.2015.08.018. Epub 2015 Sep 28.

Abstract

Background: Viral infection is one of the major causes of mortality in patients undergoing hematopoietic stem cell transplant (HSCT). Systemic infection of adenovirus (AdV) has emerged as a not uncommon viral infection with significant morbidity and mortality as with cytomegalovirus and Epstein-Barr virus infection. Routine surveillance for these viruses has become a clinical practice and subsequent preemptive therapy improves patients' outcomes; however, the effectiveness of preemptive therapy for AdV has not been fully investigated in patients with a lethal form of AdV infection.

Methods: Sequential AdV loads were retrospectively analyzed in children with fulminant AdV hepatitis after HSCT.

Results: The AdV DNA became detectable (1 × 10(4) copies/mL) as early as 2 weeks after HSCT. These levels reached >1 × 10(8) copies/mL at the onset of fulminant hepatitis. However, we determined that γ-glutamyltransferase levels were elevated to >100 IU/L at least 2 weeks before the diagnosis of hepatitis.

Conclusions: Our observation raises the possibility that elevated γ-glutamyltransferase could be a sentinel marker for AdV hepatitis, which prompts elaborated monitoring of AdV load and targeted treatment.

Keywords: Adenovirus; Bone marrow transplant; Children; Fulminant hepatitis; Real-time PCR; γ-glutamyltransferase.

Publication types

  • Case Reports

MeSH terms

  • Adenoviridae / drug effects
  • Adenoviridae / genetics*
  • Adenovirus Infections, Human / drug therapy
  • Adenovirus Infections, Human / virology*
  • Adolescent
  • Antiviral Agents / therapeutic use
  • Child
  • Cytomegalovirus / drug effects
  • Cytomegalovirus / genetics
  • DNA, Viral / genetics*
  • Female
  • Hematopoietic Stem Cell Transplantation
  • Hepatitis / drug therapy
  • Hepatitis / virology*
  • Humans
  • Real-Time Polymerase Chain Reaction / methods
  • Retrospective Studies
  • Viral Load / drug effects
  • Viral Load / methods
  • Virus Diseases / drug therapy
  • Virus Diseases / virology

Substances

  • Antiviral Agents
  • DNA, Viral