Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae

Daniel Golparian; Stina Boräng; Martin Sundqvist; Magnus Unemo

doi:10.1128/JCM.01962-15

Evaluation of the New BD Max GC Real-Time PCR Assay, Analytically and Clinically as a Supplementary Test for the BD ProbeTec GC Qx Amplified DNA Assay, for Molecular Detection of Neisseria gonorrhoeae

J Clin Microbiol. 2015 Dec;53(12):3935-7. doi: 10.1128/JCM.01962-15. Epub 2015 Oct 14.

Authors

Daniel Golparian¹, Stina Boräng², Martin Sundqvist¹, Magnus Unemo³

Affiliations

¹ WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
² Department of Clinical Microbiology, Karolinska University Hospital, Huddinge, Sweden.
³ WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden magnus.unemo@regionorebrolan.se.

Abstract

The new BD Max GC real-time PCR assay showed high clinical and analytical sensitivity and specificity. It can be an effective and accurate supplementary test for the BD ProbeTec GC Qx amplified DNA assay, which had suboptimal specificity, and might also be used for initial detection of Neisseria gonorrhoeae.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Female
Gonorrhea / diagnosis*
Humans
Male
Molecular Diagnostic Techniques / methods*
Neisseria gonorrhoeae / genetics
Neisseria gonorrhoeae / isolation & purification*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity