Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA

Kristen L Kozielski; Jordan J Green

doi:10.1007/978-1-4939-3112-5_8

Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of SiRNA

Methods Mol Biol. 2016:1364:79-87. doi: 10.1007/978-1-4939-3112-5_8.

Authors

Kristen L Kozielski^{1

2}, Jordan J Green^{3

4

5

6

7}

Affiliations

¹ Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
² Translational Tissue Engineering Center and Institute for Nanobiotechnology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
³ Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA. green@jhu.edu.
⁴ Translational Tissue Engineering Center and Institute for Nanobiotechnology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. green@jhu.edu.
⁵ Department of Materials Science and Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA. green@jhu.edu.
⁶ Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. green@jhu.edu.
⁷ Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. green@jhu.edu.

Abstract

RNA interference (RNAi) is a powerful tool to target and knockdown gene expression in a sequence-specific manner. RNAi can be achieved by the intracellular introduction of SiRNA; however, intracellular SiRNA delivery remains a challenging obstacle. Herein we describe the use of bioreducible nanoparticles formed using poly(beta-amino ester)s (PBAEs) for safe and efficient SiRNA delivery. Methods for polymer synthesis, nanoparticle formation, and SiRNA delivery using these particles are described. A template protocol for nanoparticle screening is presented and can be used to quickly optimize SiRNA delivery for novel applications.

Keywords: Gene delivery; Nanoparticle; Polymer; RNA interference; SiRNA; Transfection.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Cell Line, Tumor
Gene Knockdown Techniques
Humans
Intracellular Space / metabolism*
Oxidation-Reduction
Polymers / chemical synthesis
Polymers / chemistry*
Polymers / metabolism*
RNA, Small Interfering / chemistry*
RNA, Small Interfering / genetics

Substances

Polymers
RNA, Small Interfering
poly(beta-amino ester)

Abstract

Publication types

MeSH terms

Substances

Grants and funding