Although it is more than a decade since the parasite genome information was obtained, standardized novel genome-wide selection/prioritization strategies for candidacy of malaria vaccine antigens are still sought. In the quest to systematically identify candidates, it is impossible to overemphasize the usefulness of wheat germ cell-free technology in expressing quality proteins for the post-genome vaccine candidate discovery.
Keywords: Plasmodium falciparum; RTSS/AS01; antibody profiling approach; malaria; post-genome; reverse vaccinology; subunit vaccine; vaccine candidate discovery; whole-parasite vaccine.