LC-MS/MS determination of tralopyril in water samples

Isabel B Oliveira; René Schönenberger; Carlos M Barroso; Marc J-F Suter

doi:10.1016/j.chemosphere.2015.11.098

LC-MS/MS determination of tralopyril in water samples

Chemosphere. 2016 Feb:145:445-9. doi: 10.1016/j.chemosphere.2015.11.098. Epub 2015 Dec 13.

Authors

Isabel B Oliveira¹, René Schönenberger², Carlos M Barroso³, Marc J-F Suter⁴

Affiliations

¹ Cesam and Biology Department of the University of Aveiro, Campus de Santiago, Aveiro, 3810-193, Portugal; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, Ueberlandstrasse 133, Dübendorf, 8600, Switzerland.
² Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, Ueberlandstrasse 133, Dübendorf, 8600, Switzerland.
³ Cesam and Biology Department of the University of Aveiro, Campus de Santiago, Aveiro, 3810-193, Portugal.
⁴ Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, Ueberlandstrasse 133, Dübendorf, 8600, Switzerland; ETH Zürich, Environmental Systems Science, Zürich, 8092, Switzerland. Electronic address: marc.suter@eawag.ch.

PMID: 26694794
DOI: 10.1016/j.chemosphere.2015.11.098

Abstract

A targeted analytical method was established to determine tralopyril (4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile) in water. This compound has been recently introduced as a biocide in ship antifouling paints, becoming a potential new environmental contaminant. The method presented here allows for the first time the direct determination of tralopyril in environmental samples without the need of a pre-concentration step. The injected sample is separated by a 30 min HPLC-gradient on a reversed phase column and the compound identified and quantified by negative ion LC-MS/MS. Tralopyril solutions in DMSO, seawater, river Glatt water and E3 medium (used for zebrafish experiments) were analysed to demonstrate the applicability of the method. The method provides good retention time reproducibility and a quantitation limit (LOQ) of 0.025 μg L(-1) for DMSO, seawater and E3 exposure medium and 0.05 μg L(-1) for river Glatt water. Calculated tralopyril half-lives were 6.1 h for seawater, 8.1 h for river Glatt water and 7.4 h for E3 medium at 18 °C.

Keywords: Antifouling biocide; Freshwater samples; Molluscicide; Seawater samples; Tralopyril.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Environmental Monitoring / methods*
Fresh Water / analysis*
Pyrroles / analysis*
Reproducibility of Results
Seawater / analysis*
Tandem Mass Spectrometry
Water Pollutants, Chemical / analysis*

Substances

Pyrroles
Water Pollutants, Chemical
4-bromo-2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile