Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

J Clin Microbiol. 1989 Aug;27(8):1854-8. doi: 10.1128/jcm.27.8.1854-1858.1989.

Abstract

Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Bacterial / analysis*
  • Antigens, Bacterial / immunology*
  • Borrelia / immunology*
  • Cross Reactions
  • Fluorescent Antibody Technique
  • Humans
  • Immunoblotting
  • Lyme Disease / diagnosis*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial