Fumigaclavine I, a new alkaloid isolated from endophyte Aspergillus terreus

Chin J Nat Med. 2015 Dec;13(12):937-41. doi: 10.1016/S1875-5364(15)30101-1.

Abstract

The present study was designed to isolate and purify chemical constituents from solid culture of endophyte Aspergillus terreus LQ, using silica gel column chromatography, gel filtration with Sephadex LH-20, and HPLC. Fumigaclavine I (1), a new alkaloid, was obtained, along with seven known compounds, including fumigaclavine C (2), rhizoctonic acid (3), monomethylsulochrin (4), chaetominine (5), spirotryprostatin A (6), asperfumoid (7), and lumichrome (8). The structure of compound 1 was elucidated by various spectroscopic analyses (UV, MS, 1D and 2D NMR). The in vitro cytotoxicity of compound 1 was determined by MTT assay in human hepatocarcinoma cell line SMMC-7721, showing weaker cytotoxicity, compared with cisplatin, a clinically used cancer chemotherapeutic agent.

Keywords: Aspergillus terreus; Chemical constituent; Endophyte; Ergot alkaloid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification
  • Aspergillus / chemistry*
  • Aspergillus / isolation & purification
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Endophytes / chemistry*
  • Endophytes / isolation & purification
  • Ergot Alkaloids / chemistry*
  • Ergot Alkaloids / isolation & purification*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Oryza / microbiology*

Substances

  • Antineoplastic Agents
  • Ergot Alkaloids
  • fumigaclavine I