Trichoderma L-Lysine-α-Oxidase Producer Strain Culture Fluid Inhibits Impatiens Necrotic Spot Virus

I P Smirnova; Yu A Shneider; E V Karimova

doi:10.1007/s10517-016-3170-3

Trichoderma L-Lysine-α-Oxidase Producer Strain Culture Fluid Inhibits Impatiens Necrotic Spot Virus

Bull Exp Biol Med. 2016 Jan;160(3):357-9. doi: 10.1007/s10517-016-3170-3. Epub 2016 Jan 8.

Authors

I P Smirnova¹, Yu A Shneider², E V Karimova²

Affiliations

¹ Medical Faculty, Medical Institute, Russian University of Peoples Friendship, Moscow, Russia. smirip@yandex.ru.
² Medical Faculty, Medical Institute, Russian University of Peoples Friendship, Moscow, Russia.

PMID: 26742733
DOI: 10.1007/s10517-016-3170-3

Abstract

A method for PCR diagnosis of impatiens necrotic spot virus is developed. Concentrated culture fluid with active L-lysine-α-oxidase (0.54 U/ml) from Trichoderma harzianum Rifai fungus producer strain F-180 inhibits vitally hazardous impatiens necrotic spot phytovirus.

Keywords: L-lysine-α-oxidase; culture fluid; impatiens necrotic spot virus; phytopathogen.

MeSH terms

Amino Acid Oxidoreductases / metabolism*
Antiviral Agents / chemistry
Antiviral Agents / pharmacology
Tospovirus / drug effects
Trichoderma / chemistry*
Trichoderma / enzymology

Substances

Antiviral Agents
Amino Acid Oxidoreductases
L-lysine oxidase