Solid-State NMR of PEGylated Proteins

Enrico Ravera; Silvia Ciambellotti; Linda Cerofolini; Tommaso Martelli; Tatiana Kozyreva; Caterina Bernacchioni; Stefano Giuntini; Marco Fragai; Paola Turano; Claudio Luchinat

doi:10.1002/anie.201510148

Solid-State NMR of PEGylated Proteins

Angew Chem Int Ed Engl. 2016 Feb 12;55(7):2446-9. doi: 10.1002/anie.201510148. Epub 2016 Jan 12.

Affiliations

¹ Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy.
² Giotto Biotech S.r.l. Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy.
³ Magnetic Resonance Center (CERM) and Department of Chemistry "Ugo Schiff", University of Florence, and Magnetic Resonance Consortium (CIRMMP), Via L. Sacconi 6, 50019, Sesto Fiorentino, FI, Italy. claudioluchinat@cerm.unifi.it.
⁴ Giotto Biotech S.r.l. Via Madonna del Piano 6, 50019, Sesto Fiorentino, FI, Italy. claudioluchinat@cerm.unifi.it.

PMID: 26756539
DOI: 10.1002/anie.201510148

Abstract

PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic-level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid-state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use.

Keywords: PEGylated proteins; biopharmaceuticals; protein modification; protein structures; solid-state NMR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Spectroscopy / methods*
Polyethylene Glycols / chemistry*
Proteins / chemistry*

Substances

Proteins
Polyethylene Glycols