Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2

Lei Fu; Jianqing Zhao; Lan Lin; Qiang Zhang; Zhongmin Xu; Li Han; Caiyun Xie; Rui Zhou; Meilin Jin; Anding Zhang

doi:10.1016/j.micinf.2015.12.005

Characterization of IgA1 protease as a surface protective antigen of Streptococcus suis serotype 2

Microbes Infect. 2016 Apr;18(4):285-9. doi: 10.1016/j.micinf.2015.12.005. Epub 2016 Jan 14.

Authors

Lei Fu¹, Jianqing Zhao², Lan Lin³, Qiang Zhang⁴, Zhongmin Xu⁴, Li Han⁴, Caiyun Xie⁴, Rui Zhou⁵, Meilin Jin⁵, Anding Zhang⁶

Affiliations

¹ National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
² College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
³ College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
⁴ College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China.
⁵ National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, Hubei, 430070, China.
⁶ National Key Laboratory of Agricultural Microbiology, 1 Shizishan Street, Wuhan, Hubei, 430070, China; College of Veterinary Medicine, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China. Electronic address: andye8019@mail.hzau.edu.cn.

PMID: 26774332
DOI: 10.1016/j.micinf.2015.12.005

Abstract

IgA1 protease of Streptococcus suis serotype 2 (SS2) has been proven to be relative with virulence and immunogenicity, however, its protective efficacy remained to be evaluated. The present study found evidence that immunization with purified recombinant IgA1 protease (600-1926aa) could induce high IgG antibody titers and could confer complete protection against a challenge with a lethal dose of SS2 in a mouse model. In addition, our findings confirmed that the IgA1 protease distributes on the surface of SS2. Therefore, the present study identified the virulence-associated protein, IgA1 protease, as a novel surface protective antigen of SS2.

Keywords: IgA1 protease; Protective antigen; Streptococcus suis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Bacterial / blood*
Antigens, Bacterial / immunology*
Disease Models, Animal
Membrane Proteins / immunology
Mice, Inbred BALB C
Serine Endopeptidases / immunology*
Serogroup
Streptococcal Infections / immunology
Streptococcal Infections / prevention & control*
Streptococcal Vaccines / administration & dosage
Streptococcal Vaccines / genetics
Streptococcal Vaccines / immunology*
Streptococcus suis / classification
Streptococcus suis / enzymology*
Streptococcus suis / immunology*
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / genetics
Vaccines, Synthetic / immunology

Substances

Antibodies, Bacterial
Antigens, Bacterial
Membrane Proteins
Streptococcal Vaccines
Vaccines, Synthetic
Serine Endopeptidases
IgA-specific serine endopeptidase