Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India

Biljo V Joseph; Smitha Soman; Véronique Hill; R Ajay Kumar; Nalin Rastogi; Sathish Mundayoor

doi:10.1016/j.ijmyco.2013.10.005

Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India

Int J Mycobacteriol. 2013 Dec;2(4):244-7. doi: 10.1016/j.ijmyco.2013.10.005. Epub 2013 Nov 2.

Authors

Biljo V Joseph¹, Smitha Soman², Véronique Hill³, R Ajay Kumar², Nalin Rastogi³, Sathish Mundayoor⁴

Affiliations

¹ Department of Biotechnology, Christ University, Hosur Road, Bangalore 560 029, Karnataka, India.
² Mycobacteria Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram 695 014, Kerala, India.
³ WHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, Abymes, France.
⁴ Mycobacteria Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thycaud Post, Thiruvananthapuram 695 014, Kerala, India. Electronic address: smundayoor@rgcb.res.in.

PMID: 26786131
DOI: 10.1016/j.ijmyco.2013.10.005

Abstract

The present study evaluated the ability of MIRU-VNTRs to discriminate Mycobacterium tuberculosis (MTB) clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping (discriminatory index of 0.9113) differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 (n=48). The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates (discriminatory indexes of 0.7819 and 0.5523, respectively).

Keywords: IS6110-RFLP; India; Kerala; MIRU-VNTR; Mycobacterium tuberculosis; Spoligotyping.