Click-Crosslinked Injectable Gelatin Hydrogels

Sandeep T Koshy; Rajiv M Desai; Pascal Joly; Jianyu Li; Rishi K Bagrodia; Sarah A Lewin; Neel S Joshi; David J Mooney

doi:10.1002/adhm.201500757

Click-Crosslinked Injectable Gelatin Hydrogels

Adv Healthc Mater. 2016 Mar 9;5(5):541-7. doi: 10.1002/adhm.201500757. Epub 2016 Jan 25.

Authors

Sandeep T Koshy^{1

2

3}, Rajiv M Desai^{1

2}, Pascal Joly^{1

4}, Jianyu Li^{1

2}, Rishi K Bagrodia^{1

2}, Sarah A Lewin¹, Neel S Joshi^{1

2}, David J Mooney^{1

2}

Affiliations

¹ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
² John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
³ Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, 02139, USA.
⁴ Julius Wolff Institute and Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, 13353, Germany.

Abstract

Injectable gelatin hydrogels formed with bioorthogonal click chemistry (ClickGel) are cell-responsive ECM mimics for in vitro and in vivo biomaterials applications. Gelatin polymers with pendant norbornene (GelN) or tetrazine (GelT) groups can quickly and spontaneously crosslink upon mixing, allowing for high viability of encapsulated cells, establishment of 3D elongated cell morphologies, and biodegradation when injected in vivo.

Keywords: click chemistry; degradable; gelatin; hydrogels; injectable.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

3T3 Cells
Animals
Cell Adhesion
Cell Proliferation
Cell Shape
Click Chemistry / methods*
Cross-Linking Reagents / chemistry*
Female
Gelatin / chemistry*
Hydrogels / chemistry*
Mice
Subcutaneous Tissue

Substances

Cross-Linking Reagents
Hydrogels
Gelatin

Abstract

Publication types

MeSH terms

Substances

Grants and funding