Evaluation of GenoFlow DR-MTB Array Test for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis

J Clin Microbiol. 2016 Apr;54(4):1160-3. doi: 10.1128/JCM.03341-15. Epub 2016 Feb 10.

Abstract

The aim of this study was to evaluate the GenoFlow DR-MTB array test (DiagCor Bioscience, Hong Kong) on 70 cultured isolates and 50 sputum specimens. The GenoFlow array test showed good sensitivity and specificity compared to the phenotypic Bactec 460TB. This array accurately detected mutations inrpoB,katG, andinhAassociated with resistance to rifampin and isoniazid.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Catalase / genetics
  • Drug Resistance, Bacterial*
  • Genotype
  • Genotyping Techniques / methods*
  • Humans
  • Isoniazid / pharmacology*
  • Mutation
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / genetics*
  • Oxidoreductases / genetics
  • RNA Polymerase II / genetics
  • Rifampin / pharmacology*
  • Sensitivity and Specificity

Substances

  • Antitubercular Agents
  • Bacterial Proteins
  • Oxidoreductases
  • Catalase
  • katG protein, Mycobacterium tuberculosis
  • InhA protein, Mycobacterium
  • RNA Polymerase II
  • Isoniazid
  • Rifampin

Grants and funding

J. Domínguez is funded by the Miguel Servet program of the Instituto de Salud Carlos III (Spain). The research was partially supported by a grant from the Instituto de Salud Carlos III (PI 13/01546). The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.