Whole-Heart Construct Cultivation Under 3D Mechanical Stimulation of the Left Ventricle

Methods Mol Biol. 2016:1502:181-94. doi: 10.1007/7651_2015_317.

Abstract

Today the concept of Whole-Heart Tissue Engineering represents one of the most promising approaches to the challenge of synthesizing functional myocardial tissue. At the current state of scientific and technological knowledge it is a principal task to transfer findings of several existing and widely investigated models to the process of whole-organ tissue engineering. Hereby, we present the first bioreactor system that allows the integrated 3D biomechanical stimulation of a whole-heart construct while allowing for simultaneous controlled perfusion of the coronary system.

Keywords: 3D stretching; Biomechanical stimulation; Myocardial tissue engineering; Whole heart bioreactor; Whole-organ tissue engineering.

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Bioreactors*
  • Cell Survival
  • Coronary Vessels / cytology*
  • Coronary Vessels / physiology
  • Equipment Design
  • Heart / physiology*
  • Heart Ventricles / cytology*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Male
  • Myocardium / cytology
  • Myocardium / metabolism
  • Organ Culture Techniques / instrumentation
  • Organ Culture Techniques / methods*
  • Perfusion / instrumentation
  • Perfusion / methods
  • Rats, Wistar
  • Stress, Mechanical
  • Tensile Strength
  • Tissue Engineering / instrumentation
  • Tissue Engineering / methods*