Growth Hormone Receptor Deficiency Protects against Age-Related NLRP3 Inflammasome Activation and Immune Senescence

Cell Rep. 2016 Feb 23;14(7):1571-1580. doi: 10.1016/j.celrep.2016.01.044. Epub 2016 Feb 11.

Abstract

The hallmarks of age-related immune senescence are chronic inflammation, aberrant expansion of effector memory, and loss of naive T lymphocytes due in part to systemic activation of innate immune sensor NLRP3 inflammasome in myeloid lineage cells. The endogenous mechanisms that regulate inflammasome activation during aging are unknown. Here, we present evidence that growth hormone receptor (GH-R)-dependent downregulation of NLRP3 inflammasome in macrophages is linked to pro-longevity effects that maintain immune system homeostasis in aging. Deletion of GH-R prevented the macrophage-driven age-related activation of inflammasome in response to NLRP3 ligands and also increased the preservation of naive T cells, even in advanced age and with higher IFNγ secretion from effector cells. The mechanism of inflammasome inhibition is linked to autocrine somatotropic axis as ablation of IGF1R in macrophages lowered the NLRP3 inflammasome activation. Together, our findings show that functional somatotropic axis in macrophages controls inflammation, thus linking NLRP3-mediated innate immune signaling to health span and longevity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / genetics*
  • Aging / immunology
  • Animals
  • Autocrine Communication
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / immunology
  • Carrier Proteins / genetics*
  • Carrier Proteins / immunology
  • Gene Expression Regulation
  • Homeostasis / immunology
  • Immunity, Innate
  • Immunologic Memory
  • Inflammasomes / genetics*
  • Inflammasomes / immunology
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / immunology
  • Longevity / genetics
  • Longevity / immunology
  • Macrophages / cytology
  • Macrophages / immunology*
  • Mice
  • Mice, Knockout
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Receptor, IGF Type 1 / deficiency
  • Receptor, IGF Type 1 / genetics*
  • Receptor, IGF Type 1 / immunology
  • Receptors, Somatotropin / deficiency
  • Receptors, Somatotropin / genetics*
  • Receptors, Somatotropin / immunology
  • Signal Transduction
  • Spleen / cytology
  • Spleen / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology

Substances

  • Carrier Proteins
  • Inflammasomes
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, mouse
  • Receptors, Somatotropin
  • Interferon-gamma
  • Receptor, IGF Type 1