Nonculture Diagnostics in Fungal Disease

Abstract

Fungal diagnostics that utilize antibody, antigen or nucleic acid detection offer several advantages that supplement traditional culture-based methods. As a group, nonculture assays can help identify patients with invasive fungal infection (IFI) sooner than is possible with culture, are often more sensitive, and can be used to guide early interventions. Challenges associated with these techniques include the possibility for contamination or cross-reactivity as well as the potential for false negative tests. This review summarized the test characteristics and clinical utility of nonculture-based laboratory methods.

Keywords: Clinical mycology; Fungal antigen detection; Fungal serology; Laboratory diagnosis of invasive fungal infection; Molecular detection of fungi.