MdSOS2L1 forms a complex with MdMYB1 to control vacuolar pH by transcriptionally regulating MdVHA-B1 in apples

Plant Signal Behav. 2016;11(3):e1146846. doi: 10.1080/15592324.2016.1146846.

Abstract

Vacuolar pH is important and involves in many different physiological processes in plants. A recent paper published in Plant Physiology reveals that MdMYB1 regulates vacuolar pH by directly transcriptionally regulating proton pump genes and malate transporters genes, such as V-ATPase subunit gene MdVHA-B1. Here, we found that MdSOS2L1 in vitro did not directly interact with MdMYB1, however, in vivo formed a complex with MdMYB1 in the nucleus to regulate MdVHA-B1-mediated vacuolar acidification. This finding shed light on the role of MdSOS2L1 in transcriptionally regulating MdVHA-B1 in addition to its post-modified function in apples.

Keywords: Apple; MdMYB1; MdSOS2L1; MdVHA-B1; vacuolar pH.

MeSH terms

  • Biological Transport
  • Gene Expression Regulation, Plant
  • Hydrogen-Ion Concentration
  • Malates / metabolism*
  • Malus / chemistry
  • Malus / metabolism*
  • Phosphorylation
  • Plant Proteins / analysis
  • Plant Proteins / metabolism
  • Plant Proteins / physiology*
  • Protein Kinases / analysis
  • Protein Kinases / physiology*
  • Recombinant Fusion Proteins / analysis
  • Signal Transduction*

Substances

  • Malates
  • Plant Proteins
  • Recombinant Fusion Proteins
  • malic acid
  • Protein Kinases