Non-enzymatic amperometric detection of hydrogen peroxide in human blood serum samples using a modified silver nanowire electrode

J Colloid Interface Sci. 2016 May 15:470:117-122. doi: 10.1016/j.jcis.2016.02.049. Epub 2016 Feb 21.

Abstract

In this paper, we report a highly sensitive amperometric H2O2 sensor based on silver nanowires (AgNWs) modified screen printed carbon electrode. The AgNWs were synthesized using polyol method. The synthesized AgNWs were characterized by scanning electron microscopy, UV-vis spectroscopy and X-ray diffraction techniques. The average diameter and length of the synthesized AgNWs were found as 86±5 and 385nm, respectively. Under optimum conditions, the AgNWs modified electrode shows a stable amperometric response for H2O2 and was linear over the concentrations ranging from 0.3 to 704.8μM. The non-enzymatic sensor showed a high sensitivity of 662.6μAmM(-1)cm(-2) with a detection limit of 29nM. The response time of the sensor was found as 2s. Furthermore, the AgNWs modified electrode exhibited a good recovery of H2O2 (94.3%) in the human blood serum samples.

Keywords: Hydrogen peroxide; Non-enzymatic sensor; Screen printed carbon electrode; Silver nanowires.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrodes
  • Humans
  • Hydrogen Peroxide / blood*
  • Nanowires / chemistry*
  • Particle Size
  • Silver / chemistry*
  • Surface Properties

Substances

  • Silver
  • Hydrogen Peroxide