In the soil samples taken from different places of Bursa, distribution of the Clostridia were searched by anaerobic jar and immunofluorescence reaction (FAT). In our study, 122 bacteria belonging to 11 Clostridium species were isolated in 35 soil samples. These strains were identified by studying morphological and biochemical properties, lecithinase C and lipase activities, toxin neutralization characteristics. In addition, FAT were used for 4 Clostridium species.