Novel AVPR2 mutation causing partial nephrogenic diabetes insipidus in a Japanese family

J Pediatr Endocrinol Metab. 2016 May 1;29(5):591-6. doi: 10.1515/jpem-2015-0323.

Abstract

Background: X-linked recessive congenital nephrogenic diabetes insipidus (NDI) is caused by mutations of the arginine vasopressin type 2 receptor gene (AVPR2). More than 200 mutations of the AVPR2 gene with complete NDI have been reported although only 15 mutations with partial NDI has been reported to date.

Methods: We herein report a Japanese kindred with partial NDI. The proband is an 8-year-old boy who was referred to our hospital for nocturnal enuresis. Water deprivation test and hypertonic saline test suggested partial renal antidiuretic hormone arginine vasopressin (AVP) resistance.

Results: Analysis of genomic DNA revealed a novel missense mutation (p.L161P) in the patient. The patient's mother was heterozygous for the mutation. Three-dimensional (3-D) modeling study showed that L161P possibly destabilizes the transmembrane domain of the V2 receptor, resulting in its misfolding or mislocalization.

Conclusions: Distinguishing partial NDI from nocturnal enuresis is important. A clinical clue for diagnosis of partial NDI is an incompatibly high level of AVP despite normal serum osmolality.

Publication types

  • Case Reports

MeSH terms

  • Biomarkers / metabolism
  • Child
  • DNA Mutational Analysis / methods
  • Diabetes Insipidus, Nephrogenic / genetics*
  • Diabetes Insipidus, Nephrogenic / pathology
  • Humans
  • Japan
  • Male
  • Mutation, Missense / genetics*
  • Pedigree
  • Polymerase Chain Reaction
  • Prognosis
  • Protein Conformation
  • Receptors, Vasopressin / chemistry
  • Receptors, Vasopressin / genetics*

Substances

  • AVPR2 protein, human
  • Biomarkers
  • Receptors, Vasopressin