Objective: To investigate the protective mechanism of total saponins from Panax japonicus (SPJ) on H₂O₂ induced injury in SH-SY5Y cells.
Methods: SH-SY5Y cells were divided into three groups: blank control group, model group (600 µmol/L H₂O₂) and drug treatment groups. Different concentrations of SPJ (0.1, 1, 5 and 20 µg/mL) were incubated with SH-SY5Y cells for 12 hours prior to exposing to 600 µmol/L H₂O₂ for another 12 h. Mitochondrial membrane potential (MMP) was detected by JC-1 method. Protein expressions of Sirt1 , PGC-1α, Foxo3a, LC3-II and Beclin1 were detected by Western blotting.
Results: Compared to the H₂O₂ model group, SPJ pretreatment significantly increased MMP level and enhanced the protein expressions of Sirt1, PGC-1α, Foxo3a, LC3-II and Beclin1.
Conclusion: SPJ exerts protective effect on H₂O₂ induced SH-SY5Y cell injury through mitochondria pathway.