Generation of an iPS cell line from bone marrow derived mesenchymal stromal cells from an elderly patient

Matthias Megges; Sven Geissler; Georg N Duda; James Adjaye

doi:10.1016/j.scr.2015.10.003

Generation of an iPS cell line from bone marrow derived mesenchymal stromal cells from an elderly patient

Stem Cell Res. 2015 Nov;15(3):565-8. doi: 10.1016/j.scr.2015.10.003. Epub 2015 Oct 9.

Authors

Matthias Megges¹, Sven Geissler², Georg N Duda³, James Adjaye⁴

Affiliations

¹ Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Max Planck Institut für Molekulare Genetik, Ihnestraße 63-73, 14195 Berlin, Germany; Department of Biology, Chemistry and Pharmacy, Institute of Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
² Berlin-Brandenburger Centrum für Regenerative Therapien, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
³ Berlin-Brandenburger Centrum für Regenerative Therapien, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Julius Wolff Institut, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
⁴ Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Max Planck Institut für Molekulare Genetik, Ihnestraße 63-73, 14195 Berlin, Germany. Electronic address: James.Adjaye@med.uni-duesseldorf.de.

PMID: 26987921
DOI: 10.1016/j.scr.2015.10.003

Abstract

An induced pluripotent stem cell line was generated from primary human bone marrow derived mesenchymal stromal cells of a 74 year old donor using retroviruses harboring OCT4, SOX2, KLF4 and c-MYC in combination with the following inhibitors TGFβ receptor-SB 431542, MEK-PD325901, and p53-Pifithrin α. Pluripotency was confirmed both in vitro and in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Bone Marrow / metabolism*
Cell Differentiation
Cell Line
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4
Mesenchymal Stem Cells / metabolism*