Interferon-gamma release assays in patients with Mycobacterium kansasii pulmonary infection: A retrospective survey

J Infect. 2016 Jun;72(6):706-712. doi: 10.1016/j.jinf.2016.03.011. Epub 2016 Mar 26.

Abstract

Objectives: Interferon-gamma release assays (IGRAs) can be positive in patients infected with Mycobacterium kansasii (M. kansasii), which carries some of Mycobacterium tuberculosis specific antigens adopted for IGRAs. Our aim is to evaluate positive rate and factors associated with positive IGRAs in patients with M. kansasii pulmonary infection.

Methods: We retrospectively investigated 105 M. kansasii cases in which IGRAs were performed before or ≦14 days after treatment initiation. Clinical characteristics including a history of tuberculosis, radiographic features and laboratory data were collected from medical records.

Results: Positive rate of each IGRA was 25.9% (15/58) in QuantiFERON TB-Gold (QFT-G), 31.8% (7/22) in QuantiFERON-TB Gold In Tube (QFT-GIT), and 33.3% (7/21) in T-SPOT. TB (T-SPOT). After excluding cases having a history of tuberculosis, positive rate of each IGRA decreased to 19% (8/42) in QFT-G, 20% (3/15) in QFT-GIT, and 18.8% (3/16) in T-SPOT. The multivariate analysis revealed that only previous tuberculosis was significantly associated with positive IGRAs (odds ratio, 4.758; 95% confidence interval, 1.73-13.05; p = 0.002).

Conclusions: Positive rates of IGRAs were low in patients with M. kansasii, especially in those without previous tuberculosis. M. kansasii pulmonary infection alone might induce less interferon-gamma production with the antigens.

Keywords: Interferon-gamma release assays; Mycobacterium kansasii.

MeSH terms

  • Adult
  • Aged
  • Antigens, Bacterial / immunology
  • Female
  • Humans
  • Interferon-gamma / analysis*
  • Interferon-gamma / immunology
  • Interferon-gamma Release Tests*
  • Latent Tuberculosis / immunology
  • Latent Tuberculosis / microbiology
  • Lung / microbiology
  • Male
  • Middle Aged
  • Mycobacterium Infections, Nontuberculous / immunology*
  • Mycobacterium Infections, Nontuberculous / microbiology
  • Mycobacterium kansasii / immunology
  • Mycobacterium kansasii / isolation & purification*
  • Mycobacterium tuberculosis / immunology
  • Mycobacterium tuberculosis / isolation & purification
  • Retrospective Studies
  • Sensitivity and Specificity
  • Surveys and Questionnaires
  • Tuberculin Test
  • Tuberculosis / immunology*

Substances

  • Antigens, Bacterial
  • Mycobacterium tuberculosis antigens
  • Interferon-gamma