Effect of serum 25-hydroxyvitamin D3 on insulin resistance and β-cell function in newly diagnosed type 2 diabetes patients

J Diabetes Investig. 2016 Mar;7(2):226-32. doi: 10.1111/jdi.12381. Epub 2015 Jul 8.

Abstract

Aims/introduction: To evaluate serum 25-hydroxyvitamin D3 (25(OH)D3) in newly diagnosed type 2 diabetes patients and to explore the associations of 25(OH)D3 with insulin resistance and β-cell function.

Materials and methods: A total of 97 newly diagnosed type 2 diabetes patients and 69 healthy controls were recruited. Serum 25(OH)D3 was determined using high-pressure liquid chromatography. Insulin resistance was measured using a homeostasis model assessment of insulin resistance (HOMA-IR). β-Cell function was determined using the HOMA β-cell function index (HOMA-β), early-phase insulin secretion index (ΔI30/ΔG30) and area under the insulin curve (AUCins). Correlation analysis was carried out using Pearson's correlation and multiple stepwise regression analysis.

Results: Serum 25(OH)D3 was much lower in patients with newly diagnosed type 2 diabetes (t = -13.00, P < 0.01), and the prevalence of hypovitaminosis 25(OH)D3 was 62.9% (61/97) in diabetic patients. Among the diabetic patients, patients with hypovitaminosis 25(OH)D3 showed higher glycosylated hemoglobin and AUCglu (P < 0.01) as well as lower HOMA-β, ΔI30/ΔG30 and AUCins. Serum 25(OH)D3 was independently positively correlated with ΔI30/ΔG30 and AUCins (P < 0.05), but was not significantly correlated with either HOMA-IR or HOMA-β. Only triglycerides, glycosylated hemoglobin and ΔI30/ΔG30 emerged as independent factors associated with serum 25(OH)D3 in both diabetes patients and the health control group.

Conclusions: The present results further showed a low serum 25(OH)D3 concentration in patients with newly diagnosed type 2 diabetes. 25(OH)D3 deficiency is associated with disturbances in glucose metabolism and lipid metabolism. Serum 25(OH)D3 is not correlated with basal insulin resistance or β-cell function, but is significantly positively correlated with glucose-stimulated insulin secretion and β-cell function.

Keywords: 25‐Hydroxyvitamin D3; Insulin resistance; Newly diagnosed type 2 diabetes.

MeSH terms

  • Calcifediol / blood*
  • Chromatography, High Pressure Liquid
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / metabolism*
  • Diabetes Mellitus, Type 2 / pathology
  • Humans
  • Insulin Resistance*
  • Insulin-Secreting Cells / physiology*
  • Regression Analysis

Substances

  • Calcifediol