Self-Assembly of the Cyclic Lipopeptide Daptomycin: Spherical Micelle Formation Does Not Depend on the Presence of Calcium Chloride

Steven Kirkham; Valeria Castelletto; Ian William Hamley; Katsuaki Inoue; Robert Rambo; Mehedi Reza; Janne Ruokolainen

doi:10.1002/cphc.201600308

Self-Assembly of the Cyclic Lipopeptide Daptomycin: Spherical Micelle Formation Does Not Depend on the Presence of Calcium Chloride

Chemphyschem. 2016 Jul 18;17(14):2118-22. doi: 10.1002/cphc.201600308. Epub 2016 Apr 18.

Authors

Steven Kirkham¹, Valeria Castelletto¹, Ian William Hamley², Katsuaki Inoue³, Robert Rambo³, Mehedi Reza⁴, Janne Ruokolainen⁴

Affiliations

¹ Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6AD, UK.
² Department of Chemistry, University of Reading, Whiteknights, Reading, RG6 6AD, UK. I.W.Hamley@reading.ac.uk.
³ Diamond Light Source, Harwell Science and Innovation Campus, Fermi Avenue, Didcot, OX11 0DE, UK.
⁴ Dep. of Applied Physics, Aalto University, PO Box 15100, 00076, Aalto, Finland.

PMID: 27043447
DOI: 10.1002/cphc.201600308

Abstract

The cyclic lipopeptide Daptomycin, used as a treatment for infections where antimicrobial resistance is observed, is shown to self-assemble into spherical micelles above a critical aggregation concentration. Micelles are observed either in the absence or presence of CaCl2 , in contrast to claims in the literature that CaCl2 is required for micellization.

Keywords: antimicrobial peptides; lipopeptide; micelles; secondary structure; self-assembly.

MeSH terms

Calcium Chloride / chemistry*
Circular Dichroism
Cryoelectron Microscopy
Daptomycin / chemistry*
Lipopeptides / chemistry
Micelles*
Molecular Conformation
Peptides, Cyclic / chemistry
Scattering, Small Angle
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Lipopeptides
Micelles
Peptides, Cyclic
Calcium Chloride
Daptomycin