Identification of novel and diverse rotaviruses in rodents and insectivores, and evidence of cross-species transmission into humans

Virology. 2016 Jul:494:168-77. doi: 10.1016/j.virol.2016.04.017. Epub 2016 Apr 26.

Abstract

Rotaviruses are an important cause of severe diarrheal illness in children globally. We characterized rotaviruses sampled in humans, insectivores (shrews) and rodents from urban and rural regions of Zhejiang province, China. Phylogenetic analyses revealed seven genotypic constellations of human rotaviruses with six different combinations of G and P genotypes - G3P[8] (50.06%), G9P[8] (36.16%), G1P[8] (8.92%), G2P[4] (4.63%), G3P[3] (0.12%), and G3P[9] (0.12%). In rodents and shrews sampled from the same locality we identified a novel genotype constellation (G32-P[46]-I24-R18-C17-M17-A28-N17-T19-E24-H19), a novel P genotype (P[45]), and two different AU-1-like rotaviruses associated with a G3P[3] genotype combination. Of particular note was a novel rotavirus from a human patient that was closely related to viruses sampled from rodents in the same region, indicative of a local species jump. In sum, these data are suggestive of the cross-species transmission of rodent rotaviruses into humans and for reassortment among human and animal rotaviruses.

Keywords: Evolution; Genotype; Human infection; Reassortment; Rodents; Rotavirus; Shrews.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capsid Proteins / genetics
  • Child
  • Child, Preschool
  • China / epidemiology
  • Diarrhea / virology
  • Evolution, Molecular
  • Feces / virology
  • Female
  • Genome, Viral
  • Genotype
  • Humans
  • Infant
  • Insecta
  • Male
  • Mammals
  • Phylogeny
  • Rodentia
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / transmission*
  • Rotavirus Infections / virology*
  • Zoonoses / transmission
  • Zoonoses / virology

Substances

  • Capsid Proteins