Retinyl Palmitate Supplementation Modulates T-bet and Interferon Gamma Gene Expression in Multiple Sclerosis Patients

J Mol Neurosci. 2016 Jul;59(3):360-5. doi: 10.1007/s12031-016-0747-2. Epub 2016 Apr 28.

Abstract

Vitamin A derivatives such as retinoic acid may improve the impaired balance of CD4+ T cells in autoimmune and inflammatory diseases. This study is a double-blind randomized trial to evaluate the effect of vitamin A (as form of retinyl palmitate) supplementation on multiple sclerosis (MS) patients. Thirty-nine patients were enrolled and randomly assigned to two groups. Both groups were followed for 6 months. The experimental group received 25,000 IU of retinyl palmitate daily, while the control group received a placebo. Before and after the study, the expression of interferon gamma (IFN-γ) and T-bet genes was evaluated in peripheral blood mononuclear cells of patients by RT-PCR. The results showed that after 6 months of supplementation, expression of IFN-γ and T-bet was significantly decreased. These data suggest that retinyl palmitate supplementation can modulate the impaired balance of Th1 and Th2 cells and vitamin A products that may be involved in the therapeutic mechanism of vitamin A in MS patients. This study provides information regarding the decreased gene expression of IFN-γ and T-bet in MS by retinyl palmitate supplementation.

Keywords: IFN-γ; Multiple sclerosis; Retinyl palmitate; T-bet.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Diterpenes
  • Female
  • Humans
  • Interferon-gamma / blood*
  • Male
  • Multiple Sclerosis, Relapsing-Remitting / blood
  • Multiple Sclerosis, Relapsing-Remitting / drug therapy*
  • Retinyl Esters
  • T-Box Domain Proteins / blood*
  • Th1 Cells / drug effects
  • Th1 Cells / metabolism
  • Th2 Cells / drug effects
  • Th2 Cells / metabolism
  • Vitamin A / administration & dosage
  • Vitamin A / adverse effects
  • Vitamin A / analogs & derivatives*
  • Vitamin A / pharmacology
  • Vitamin A / therapeutic use
  • Vitamins / administration & dosage
  • Vitamins / adverse effects
  • Vitamins / pharmacology*
  • Vitamins / therapeutic use

Substances

  • Diterpenes
  • Retinyl Esters
  • T-Box Domain Protein 2
  • T-Box Domain Proteins
  • Vitamins
  • Vitamin A
  • retinol palmitate
  • Interferon-gamma