In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification

Lichun Tang; Mengmeng Gong; Pumin Zhang

doi:10.1016/j.bbrc.2016.04.129

In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification

Biochem Biophys Res Commun. 2016 May 27;474(2):395-399. doi: 10.1016/j.bbrc.2016.04.129. Epub 2016 Apr 26.

Authors

Lichun Tang¹, Mengmeng Gong², Pumin Zhang³

Affiliations

¹ State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China. Electronic address: 1212qq-@163.com.
² State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China.
³ State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, China; National Center for Protein Sciences Beijing, Life Sciences Park, Beijing 102206, China.

PMID: 27125457
DOI: 10.1016/j.bbrc.2016.04.129

Abstract

The CRISPR-Cas9 genome editing system has been widely used in multiple cells and organisms. Here we developed a CRISPR-Cas9 based in vitro large DNA vector editing system, using the Ad5-based vector as an example. We demonstrate use of this system to generate targeted mutations, in-frame gene deletion, and gene replacement. This in vitro CRISPR editing system exhibits high efficiency and accuracy. We believe this system can be applied in a variety of experimental settings.

Keywords: Ad5/F35; Adenovirus; CRISPR-Cas9; Fiber modification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae / genetics*
CRISPR-Cas Systems / genetics*
Gene Editing / methods*
Genetic Vectors / genetics
Mutagenesis, Site-Directed / methods*
Transfection / methods*

Abstract

Publication types

MeSH terms

Grants and funding