Atomic-level analysis of membrane-protein structure

Nat Struct Mol Biol. 2016 Jun 7;23(6):464-7. doi: 10.1038/nsmb.3215.

Abstract

Membrane proteins are substantially more challenging than natively soluble proteins as subjects for structural analysis. Thus, membrane proteins are greatly underrepresented in structural databases. Recently, focused consortium efforts and advances in methodology for protein production, crystallographic analysis and cryo-EM analysis have accelerated the pace of atomic-level structure determination of membrane proteins.

Publication types

  • Review

MeSH terms

  • Cryoelectron Microscopy / instrumentation
  • Cryoelectron Microscopy / methods*
  • Crystallization / instrumentation
  • Crystallization / methods*
  • Crystallography, X-Ray / instrumentation
  • Crystallography, X-Ray / methods*
  • Databases, Protein
  • Detergents / chemistry*
  • Gene Expression
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Membrane Proteins / ultrastructure*
  • Models, Molecular
  • Protein Conformation
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / ultrastructure
  • Solubility

Substances

  • Detergents
  • Membrane Proteins
  • Recombinant Proteins