Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

Stem Cell Res. 2016 Jan;16(1):37-9. doi: 10.1016/j.scr.2015.12.003. Epub 2015 Dec 3.

Abstract

The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676+3A>T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Line / cytology*
  • Female
  • Human Embryonic Stem Cells / cytology*
  • Humans
  • Male
  • Mutation / genetics*
  • Pedigree
  • Von Hippel-Lindau Tumor Suppressor Protein / genetics*

Substances

  • Biomarkers
  • Von Hippel-Lindau Tumor Suppressor Protein
  • VHL protein, human