Generation of KCL037 clinical grade human embryonic stem cell line

Cristian Miere; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.023

Generation of KCL037 clinical grade human embryonic stem cell line

Stem Cell Res. 2016 Jan;16(1):149-51. doi: 10.1016/j.scr.2015.12.023. Epub 2015 Dec 29.

Authors

Cristian Miere¹, Victoria Wood¹, Neli Kadeva¹, Glenda Cornwell¹, Stefano Codognotto¹, Emma Stephenson¹, Dusko Ilic²

Affiliations

¹ Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
² Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom. Electronic address: dusko.ilic@kcl.ac.uk.

Abstract

The KCL037 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism
Blastocyst / cytology
Cell Line
Cell Survival
Comparative Genomic Hybridization
Genotype
Histocompatibility Testing
Human Embryonic Stem Cells / cytology*
Humans
Male
Microscopy, Fluorescence
Middle Aged

Substances

Alkaline Phosphatase

Abstract

Publication types

MeSH terms

Substances

Grants and funding