Introduction: This paper reports, for the first time, the presence of Rhodnius stali in the state of Acre and in the Brazilian Amazon.
Methods: Specimens of R. stali were collected by the Federal University of Acre in Rio Branco.
Results: The number of Triatominae species in the State of Acre increased from five to six. This was also the first report of R. stali in the Brazilian Amazon.
Conclusions: The occurrence of R. stali is worrisome, since this species has been found naturally infected by Trypanosoma cruzi and there has been evidence of its domiciliation capabilities.