Innate phagocytosis by peripheral blood monocytes is altered in Alzheimer's disease

Acta Neuropathol. 2016 Sep;132(3):377-89. doi: 10.1007/s00401-016-1596-3. Epub 2016 Jul 13.

Abstract

Sporadic Alzheimer's disease (AD) is characterised by the deposition and accumulation of specific protein aggregates. Failure of clearance could underlie this process, and recent genetic association studies point towards involvement of the phagocytosis and autophagy pathways. We developed a real-time tri-color flow cytometry method to quantitate the phagocytic function of human peripheral blood monocyte subsets including non-classic CD14(dim)CD16(+), intermediate CD14(+)CD16(+) and classic CD14(+)CD16(-) monocytes. Using this method, we have measured the phagocytic ability of fresh monocytes in a study of preclinical, prodromal and clinical AD, matched with cognitively normal healthy control subjects. Basal levels of phagocytosis in all three subsets of monocytes were similar between healthy controls and AD patients, while a significant increase of basal phagocytosis was found in subjects with high Aβ-amyloid burden as assessed by PET scans. Pre-treating cells with Copaxone (CPX, to stimulate phagocytosis) or ATP (an inhibitor of P2X7-mediated phagocytosis) showed a differential response depending on clinical or Aβ-burden status, indicating a relative functional deficit. Overall the results are consistent with a perturbation of basal and stimulated innate phagocytosis in sporadic AD.

Keywords: ATP; Alzheimer’s disease; Copaxone (glatiramer acetate); P2X7; Phagocytosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / immunology
  • Alzheimer Disease / metabolism*
  • Amyloidogenic Proteins / blood
  • Female
  • Humans
  • Male
  • Middle Aged
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Phagocytosis / immunology*
  • Receptors, IgG / immunology
  • Receptors, IgG / metabolism

Substances

  • Amyloidogenic Proteins
  • Receptors, IgG