Abstract
A strategy is designed for sensitive detection of tumor biomarker survivin mRNA based on resonance Rayleigh scattering of a single AuNP nanohalo probe that couples large gold nanoparticles (L-AuNPs, 52 nm) with small AuNPs (S-AuNPs, 18 nm) through the affinity interaction between streptavidin and biotin. This core-satellite plasmon ruler is further applied to imaging survivin mRNA in living cells.
MeSH terms
-
Biotin / chemistry
-
Biotin / metabolism
-
Gold / chemistry
-
HeLa Cells
-
Humans
-
Inhibitor of Apoptosis Proteins / genetics*
-
Metal Nanoparticles / chemistry*
-
Particle Size
-
RNA, Messenger / chemistry
-
RNA, Messenger / metabolism*
-
Streptavidin / chemistry
-
Streptavidin / metabolism
-
Surface Plasmon Resonance*
-
Survivin
Substances
-
BIRC5 protein, human
-
Inhibitor of Apoptosis Proteins
-
RNA, Messenger
-
Survivin
-
Biotin
-
Gold
-
Streptavidin