Structural investigation of the lipopolysaccharide O-chain isolated from Burkholderia fungorum strain DSM 17061

Antonia De Felice; Flaviana Di Lorenzo; Kirstin Scherlach; Claudia Ross; Alba Silipo; Christian Hertweck; Antonio Molinaro

doi:10.1016/j.carres.2016.07.008

Structural investigation of the lipopolysaccharide O-chain isolated from Burkholderia fungorum strain DSM 17061

Carbohydr Res. 2016 Oct 4:433:31-5. doi: 10.1016/j.carres.2016.07.008. Epub 2016 Jul 9.

Authors

Antonia De Felice¹, Flaviana Di Lorenzo¹, Kirstin Scherlach², Claudia Ross², Alba Silipo³, Christian Hertweck², Antonio Molinaro¹

Affiliations

¹ Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy.
² Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Jena, Germany; Friedrich Schiller University, Jena, Germany.
³ Department of Chemical Sciences, University of Naples Federico II, via Cinthia 4, 80126, Naples, Italy. Electronic address: silipo@unina.it.

PMID: 27434832
DOI: 10.1016/j.carres.2016.07.008

Abstract

Gram-negative bacteria exhibit lipopolysaccharides (LPSs) on their outer membrane surface. LPS is considered one of the most potent bacterial virulence factors. Here we report the elucidation of the LPS O-chain structure isolated from Burkholderia fungorum, a bacterium isolated from the white-rot fungus Phanerochaete chrysosporium that can act as a pathogen for plants and domesticated animals. The structure was determined by the employment of detailed chemical and NMR spectroscopy analyses as the following.

Keywords: Burkholderia fungorum; Lipopolysaccharide (LPS); NMR spectroscopy; O-chain; Structural characterization.

MeSH terms

Burkholderia / chemistry
Burkholderia / metabolism*
Carbohydrate Sequence
Lipopolysaccharides / chemistry
Lipopolysaccharides / isolation & purification*
Magnetic Resonance Spectroscopy
O Antigens / chemistry*

Substances

Lipopolysaccharides
O Antigens