Abstract
Features from a high throughput screening (HTS) hit and a previously reported scaffold were combined to generate 1,7-naphthyridones as novel KDM5 enzyme inhibitors with nanomolar potencies. These molecules exhibited high selectivity over the related KDM4C and KDM2B isoforms. An X-ray co-crystal structure of a representative molecule bound to KDM5A showed that these inhibitors are competitive with the co-substrate (2-oxoglutarate or 2-OG).
Keywords:
Epigenetics; Histone lysine demethylases; KDM5 inhibitors.
Copyright © 2016 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Crystallography, X-Ray
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Dogs
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Drug Design
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Humans
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Jumonji Domain-Containing Histone Demethylases / antagonists & inhibitors*
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Madin Darby Canine Kidney Cells
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Naphthyridines / chemistry
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Naphthyridines / pharmacology*
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Nuclear Proteins / antagonists & inhibitors*
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Repressor Proteins / antagonists & inhibitors*
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Retinoblastoma-Binding Protein 2 / antagonists & inhibitors*
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Structure-Activity Relationship
Substances
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Naphthyridines
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Nuclear Proteins
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Repressor Proteins
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Jumonji Domain-Containing Histone Demethylases
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KDM5A protein, human
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KDM5B protein, human
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Retinoblastoma-Binding Protein 2