A perfusion system developed for 31P NMR study of melanoma cells at tissue-like density

Magn Reson Med. 1989 Apr;10(1):96-107. doi: 10.1002/mrm.1910100109.

Abstract

A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage-dependent cells grew to tissue-like density.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Equipment Design
  • Humans
  • In Vitro Techniques
  • Magnetic Resonance Spectroscopy / methods*
  • Melanoma / analysis*
  • Melanoma / ultrastructure
  • Perfusion / instrumentation
  • Phosphorus
  • Tumor Cells, Cultured
  • Uveal Neoplasms / analysis*
  • Uveal Neoplasms / ultrastructure

Substances

  • Phosphorus