Ceftriaxone-Resistant Neisseria gonorrhoeae Isolates (2010 to 2014) in France Characterized by Using Whole-Genome Sequencing

Antimicrob Agents Chemother. 2016 Oct 21;60(11):6962-6964. doi: 10.1128/AAC.01568-16. Print 2016 Nov.

Abstract

Two extended-spectrum cephalosporin-resistant Neisseria gonorrhoeae isolates were discovered among 6,340 (0.03%) French isolates between 2010 and 2014. One isolate corresponded to the F89 multidrug-resistant N. gonorrhoeae isolate harboring a penA mosaic; whole-genome sequencing highlighted an additional R251H substitution in the ftsX gene recently involved in cephalosporin resistance. The other, ceftriaxone-resistant isolate (MIC, 0.25 mg/liter) harbored the PBP2 pattern XXXVI plus a P551S substitution and belonged to sequence type ST1579 (multilocus sequence typing [MLST]).

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Cefixime / pharmacology
  • Ceftriaxone / pharmacology*
  • Cell Cycle Proteins / genetics
  • Cephalosporin Resistance / genetics*
  • France
  • Genome, Bacterial
  • Humans
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Mutation
  • Neisseria gonorrhoeae / drug effects*
  • Neisseria gonorrhoeae / genetics*
  • Neisseria gonorrhoeae / isolation & purification

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Cell Cycle Proteins
  • FtsX protein, bacteria
  • Ceftriaxone
  • Cefixime