Novel Bovine Papillomavirus Type Discovered by Rolling-Circle Amplification Coupled with Next-Generation Sequencing

Flavio R C da Silva; Samuel P Cibulski; Cíntia Daudt; Matheus N Weber; Lorena L B Guimarães; André F Streck; Fabiana Q Mayer; Paulo M Roehe; Cláudio W Canal

doi:10.1371/journal.pone.0162345

Novel Bovine Papillomavirus Type Discovered by Rolling-Circle Amplification Coupled with Next-Generation Sequencing

PLoS One. 2016 Sep 8;11(9):e0162345. doi: 10.1371/journal.pone.0162345. eCollection 2016.

Authors

Flavio R C da Silva^{1

2}, Samuel P Cibulski¹, Cíntia Daudt¹, Matheus N Weber¹, Lorena L B Guimarães¹, André F Streck³, Fabiana Q Mayer⁴, Paulo M Roehe⁵, Cláudio W Canal¹

Affiliations

¹ Laboratório de Virologia - Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
² Centro de Ciências Biológicas e da Natureza, Universidade Federal do Acre Rio Branco, Acre, Brazil.
³ Universidade de Caxias do Sul, Caxias do Sul, Rio Grande do Sul, Brazil.
⁴ Laboratório de Biologia Molecular - Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), Fundação Estadual de Pesquisa Agropecuária, Eldorado do Sul, Eldorado do Sul, Rio Grande do Sul, Brazil.
⁵ Departamento de Microbiologia Imunologia e Parasitologia - Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.

Abstract

Currently, fifteen bovine papillomavirus (BPV) types have been identified and classified into four genera: Deltapapillomavirus, Epsilonpapillomavirus, Dyoxipapillomavirus, and Xipapillomavirus. Here, the complete genome sequence of a new BPV type (BPV 04AC14) recovered from a papillomatous lesion is reported. The genome is 7,282 bp in length and exhibits the classic genetic organization and motifs of the members of Papillomaviridae. Maximum likelihood phylogenetic analyses revealed that BPV 04AC14 clusters with members of the Xipapillomavirus genus. The nucleotide sequence of the L1 capsid protein of the novel BPV is closely related to its counterpart, BPV3, with which it shares 79% similarity. These findings suggest that this virus is a new BPV type of the Xipapillomavirus genus.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cattle
DNA, Intergenic / genetics
Epithelium / pathology
Epithelium / virology
Genome, Viral
High-Throughput Nucleotide Sequencing / methods*
Likelihood Functions
Papillomaviridae / genetics*
Papillomaviridae / isolation & purification*
Phylogeny
Polymerase Chain Reaction / methods*
Viral Proteins / chemistry
Viral Proteins / genetics

Substances

DNA, Intergenic
Viral Proteins

Grants and funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Amparo à Pesquisa do Estado do Acre (FAPAC) supported this study. CWC and PMR are CNPq productivity research fellows. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.