RYP1 gene as a target for molecular diagnosis of histoplasmosis

J Microbiol Methods. 2016 Nov:130:112-114. doi: 10.1016/j.mimet.2016.09.006. Epub 2016 Sep 13.

Abstract

This study analyzed the RYP1 gene as a target for the molecular diagnosis of histoplasmosis. This assay detected fungal DNA in 13/13 blood samples from HIV/AIDS-patients with histoplasmosis. Therefore, the detection of RYP1 gene in whole blood sample is a quick and sensitive test to diagnose histoplasmosis.

Keywords: Histoplasmosis; Molecular diagnosis; RYP1.

MeSH terms

  • Acquired Immunodeficiency Syndrome / complications
  • Base Sequence
  • Brazil
  • Coinfection / diagnosis
  • DNA, Fungal / blood
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • Gene Targeting / methods*
  • Histoplasma / genetics*
  • Histoplasma / isolation & purification
  • Histoplasmosis / diagnosis*
  • Histoplasmosis / microbiology
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Sensitivity and Specificity

Substances

  • DNA, Fungal