Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor

Eleanor A Powell; Joel E Mortensen

doi:10.1186/s12941-016-0168-7

Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor

Ann Clin Microbiol Antimicrob. 2016 Sep 22;15(1):54. doi: 10.1186/s12941-016-0168-7.

Authors

Eleanor A Powell¹, Joel E Mortensen²

Affiliations

¹ Diagnostic Infectious Disease Testing Laboratory, Cincinnati Children's Hospital Laboratory, 3333 Burnet Avenue, MLC 1010, Cincinnati, OH, 45229, USA.
² Diagnostic Infectious Disease Testing Laboratory, Cincinnati Children's Hospital Laboratory, 3333 Burnet Avenue, MLC 1010, Cincinnati, OH, 45229, USA. joel.mortensen@cchmc.org.

Abstract

The BioMerieux NucliSENS easyMAG total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. Forty eight isolates were extracted, yielding quantifiable amounts of DNA for all isolates. The easyMAG is appropriate for DNA extraction from bacterial isolates and will be incorporated in the clinical laboratory.

Keywords: 16S rRNA sequencing; Bacterial sequencing; DNA extraction.