Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40

Olivier Reynard; Mathieu Mateo

doi:10.1007/978-1-4939-3804-9_14

Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40

Methods Mol Biol. 2016:1459:205-13. doi: 10.1007/978-1-4939-3804-9_14.

Authors

Olivier Reynard¹, Mathieu Mateo²

Affiliations

¹ Inserm U1111, Université Claude Bernard Lyon 1, International Center for Infectiology Research (CIRI), 21 avenue, Tony Gamler, 69364, USA. olivier.reynard@inserm.fr.
² UBIVE, Institut Pasteur, International Center for Infectiology Research (CIRI), Paris, France.

PMID: 27665561
DOI: 10.1007/978-1-4939-3804-9_14

Abstract

While most secreted proteins use the classical endoplasmic reticulum (ER)-Golgi secretion pathway to reach the extracellular medium, a few proteins are secreted through unconventional secretary pathways. Viral proteins can be secreted through unconventional secretion pathways. Here, we describe how we have recently demonstrated that the Ebola virus (EBOV) matrix protein VP40 is released from transfected and infected cells in a soluble form through an unconventional secretion pathway.

Keywords: Ebola; Ebola virus; Unconventional secretion; VP40; Virus.

MeSH terms

Cell Death
Endoplasmic Reticulum / metabolism
Flow Cytometry
Golgi Apparatus / metabolism
HEK293 Cells
Humans
L-Lactate Dehydrogenase / metabolism
Nucleoproteins / metabolism*
Protein Transport
Secretory Pathway*
Viral Core Proteins / metabolism*

Substances

Nucleoproteins
Viral Core Proteins
nucleoprotein VP40, Ebola virus
L-Lactate Dehydrogenase