Cynomolgus monkey model of interleukin-31-induced scratching depicts blockade of human interleukin-31 receptor A by a humanized monoclonal antibody

Exp Dermatol. 2018 Jan;27(1):14-21. doi: 10.1111/exd.13236. Epub 2017 Apr 10.

Abstract

Scratching is an important factor exacerbating skin lesions through the so-called itch-scratch cycle in atopic dermatitis (AD). In mice, interleukin (IL)-31 and its receptor IL-31 receptor A (IL-31RA) are known to play a critical role in pruritus and the pathogenesis of AD; however, study of their precise roles in primates is hindered by the low sequence homologies between primates and mice and the lack of direct evidence of itch sensation by IL-31 in primates. We showed that administration of cynomolgus IL-31 induces transient scratching behaviour in cynomolgus monkeys and by that were able to establish a monkey model of scratching. We then showed that a single subcutaneous injection of 1 mg/kg nemolizumab, a humanized anti-human IL-31RA monoclonal antibody that also neutralizes cynomolgus IL-31 signalling and shows a good pharmacokinetic profile in cynomolgus monkeys, suppressed the IL-31-induced scratching for about 2 months. These results suggest that the IL-31 axis and IL-31RA axis play as critical a role in the induction of scratching in primates as in mice and that the blockade of IL-31 signalling by an anti-human IL-31RA antibody is a promising therapeutic approach for treatment of AD. Nemolizumab is currently under investigation in clinical trials.

Keywords: anti-IL-31RA antibody; atopic dermatitis; itch-scratch cycle; nemolizumab; pruritus.

MeSH terms

  • A549 Cells
  • Animals
  • Antibodies, Monoclonal, Humanized / pharmacology*
  • CHO Cells
  • Cell Line
  • Cricetulus
  • DNA, Complementary / metabolism
  • Humans
  • Interleukins / pharmacology*
  • Kinetics
  • Macaca fascicularis
  • Male
  • Mice
  • Pruritus / chemically induced*
  • Pruritus / metabolism
  • Receptors, Interleukin / metabolism*
  • Signal Transduction
  • Skin / immunology
  • Skin / pathology
  • Skin Diseases / immunology
  • Skin Diseases / pathology

Substances

  • Antibodies, Monoclonal, Humanized
  • DNA, Complementary
  • IL31 protein, human
  • IL31RA protein, human
  • Il31ra protein, mouse
  • Interleukins
  • Receptors, Interleukin
  • interleukin-31, mouse
  • nemolizumab