High-density collagen patch prevents stricture after endoscopic circumferential submucosal dissection of the esophagus: a porcine model

Gastrointest Endosc. 2017 May;85(5):1076-1085. doi: 10.1016/j.gie.2016.10.012. Epub 2016 Oct 15.

Abstract

Background and aims: Extensive excision of the esophageal mucosa by endoscopic submucosal dissection (ESD) frequently evokes a luminal stricture. This study aimed to determine the efficacy of a high-density collagen patch for the prevention of esophageal stricture in extensive ESD.

Methods: Six pigs underwent circumferential esophageal ESD under general anesthesia. In 3 pigs, artificial ulcers were covered by 2 collagen patches. The other 3 pigs underwent circumferential ESD only.

Results: The 2 collagen patches were settled onto the ulcer surface using a general endoscope and instruments. The collagen patch-treated group showed significantly better patency rates on both the oral and anal sides of the wound area compared with the control group at day 14. The mucosal re-epithelization ratio was significantly promoted, and the extent of mucosal inflammation and fibrosis was significantly decreased with the collagen patch treatment in the wound area. The frequency of cells positive α-smooth muscle actin was significantly reduced in the collagen patch-treated group compared with the control group.

Conclusions: We have established a high-density collagen device that can reduce the esophageal stricture associated with extensive ESD. This easy-to-handle device would be useful during superficial esophageal cancer treatment by ESD.

MeSH terms

  • Animals
  • Collagen / therapeutic use*
  • Endoscopic Mucosal Resection / methods*
  • Esophageal Mucosa / metabolism
  • Esophageal Mucosa / pathology
  • Esophageal Mucosa / surgery*
  • Esophageal Stenosis / prevention & control*
  • Esophagoscopy / methods*
  • Esophagus / metabolism
  • Esophagus / pathology
  • Esophagus / surgery*
  • Female
  • Gels
  • Immunohistochemistry
  • Models, Anatomic
  • Postoperative Complications / prevention & control*
  • Re-Epithelialization
  • Swine
  • Ulcer
  • Wound Healing*

Substances

  • Gels
  • atelocollagen
  • Collagen