Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)

Michael Carr; Akira Kawaguchi; Michihito Sasaki; Gabriel Gonzalez; Kimihito Ito; Yuka Thomas; Bernard M Hang'ombe; Aaron S Mweene; Guoyan Zhao; David Wang; Yasuko Orba; Akihiro Ishii; Hirofumi Sawa

doi:10.1007/s00705-016-3129-8

Isolation of a simian immunodeficiency virus from a malbrouck (Chlorocebus cynosuros)

Arch Virol. 2017 Feb;162(2):543-548. doi: 10.1007/s00705-016-3129-8. Epub 2016 Nov 1.

Authors

Michael Carr^{1

2}, Akira Kawaguchi³, Michihito Sasaki³, Gabriel Gonzalez⁴, Kimihito Ito⁴, Yuka Thomas⁵, Bernard M Hang'ombe⁶, Aaron S Mweene⁷, Guoyan Zhao⁸, David Wang⁹, Yasuko Orba³, Akihiro Ishii⁵, Hirofumi Sawa^{10

11}

Affiliations

¹ Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
² National Virus Reference Laboratory, University College Dublin, Belfield, Dublin 4, Ireland.
³ Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
⁴ Division of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan.
⁵ Hokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Lusaka, Zambia.
⁶ Department of Para-clinical studies, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
⁷ Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia.
⁸ Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
⁹ Molecular Microbiology and Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
¹⁰ Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.
¹¹ Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo, 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.

PMID: 27804019
DOI: 10.1007/s00705-016-3129-8

Abstract

To investigate the diversity of simian immunodeficiency virus (SIV) among nonhuman primates (NHPs) in Zambia, next-generation sequencing was performed to determine the complete genome sequence of a novel SIV recovered by co-culturing African green monkey (AGM) peripheral blood lymphocytes with human CD4⁺ T-cell lines. We report the first described SIV (SIVagmMAL-ZMB) from a malbrouck (Chlorocebus cynosuros). SIVagmMAL-ZMB was detected by real-time PCR analysis of splenic RNA in 3.2% (3/94) of AGMs and was undetectable in baboons (0/105). SIVagmMAL-ZMB possessed <80% nucleotide sequence identity to known SIV isolates and was located basally to vervet monkey SIV strains in all phylogenies.

MeSH terms

Animals
CD4-Positive T-Lymphocytes / virology
Cercopithecinae / virology*
Coculture Techniques
Humans
Papio
Phylogeny*
RNA, Viral / genetics*
Real-Time Polymerase Chain Reaction
Simian Acquired Immunodeficiency Syndrome / epidemiology*
Simian Acquired Immunodeficiency Syndrome / virology
Simian Immunodeficiency Virus / classification*
Simian Immunodeficiency Virus / genetics
Simian Immunodeficiency Virus / isolation & purification
Spleen / virology
Zambia / epidemiology

Substances

RNA, Viral