Multilocus sequence type profiles of Bacillus cereus isolates from infant formula in China

Food Microbiol. 2017 Apr:62:46-50. doi: 10.1016/j.fm.2016.09.007. Epub 2016 Sep 12.

Abstract

Bacillus cereus sensu stricto is an opportunistic foodborne pathogen. The multilocus sequence type (MLST) of 74 B. cereus isolated from 513 non-random infant formula in China was analyzed. Of 64 sequence types (STs) detected, 50 STs and 6 alleles were newly found in PubMLST database. All isolates except for one singleton (ST-1049), were classified into 7 clonal complexes (CC) by BURST (n-4), in which CC1 with core ancestral clone ST-26 was the largest group including 86% isolates, and CC2, 3, 9, 10 and 13 were first reported in China. MLST profiles of the isolates from 8 infant formula brands were compared. It was found the brands might be potentially tracked by the variety of STs, such as ST-1049 of singleton and ST-1062 of isolate from goat milk source, though they could not be easily tracked just by clonal complex types of the isolates.

Keywords: Bacillus cereus; Clonal complex; Infant formula; MLST; Surveillance.

MeSH terms

  • Alleles
  • Animals
  • Bacillus cereus / classification*
  • Bacillus cereus / genetics*
  • Bacillus cereus / isolation & purification
  • China
  • DNA, Bacterial
  • Genotype
  • Humans
  • Infant
  • Infant Formula / microbiology*
  • Milk / microbiology*
  • Multilocus Sequence Typing / methods
  • Product Surveillance, Postmarketing

Substances

  • DNA, Bacterial