Exome sequencing reveals germline gain-of-function EGFR mutation in an adult with Lhermitte-Duclos disease

Cold Spring Harb Mol Case Stud. 2016 Nov;2(6):a001230. doi: 10.1101/mcs.a001230.

Abstract

Lhermitte-Duclos disease (LDD) is a rare cerebellar disorder believed to be pathognomonic for Cowden syndrome. Presently, the only known etiology is germline PTEN mutation. We report a 41-yr-old white female diagnosed with LDD and wild-type for PTEN. Exome sequencing revealed a germline heterozygous EGFR mutation that breaks a disulfide bond in the receptor's extracellular domain, resulting in constitutive activation. Functional studies demonstrate activation of ERK/AKT signaling pathways, mimicking PTEN loss-of-function downstream effects. The identification of EGFR as a candidate LDD susceptibility gene contributes to advancement of molecular diagnosis and targeted therapy for this rare condition with limited treatment options.

Keywords: neoplasm of the nervous system.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Cerebellar Neoplasms / diagnosis
  • Cerebellum / metabolism
  • ErbB Receptors / genetics*
  • ErbB Receptors / metabolism
  • Exome
  • Female
  • Gain of Function Mutation / genetics
  • Ganglioneuroma / diagnosis
  • Genetic Predisposition to Disease
  • Germ Cells / metabolism
  • Germ-Line Mutation / genetics
  • Hamartoma Syndrome, Multiple / genetics*
  • Heterozygote
  • Humans
  • Mutation
  • Signal Transduction

Substances

  • EGFR protein, human
  • ErbB Receptors