Screening of Bioactive Ingredients in Ligusticum Chuanxiong Hort for Protection against Myocardial Ischemia

Cell Physiol Biochem. 2016;40(3-4):770-780. doi: 10.1159/000453137. Epub 2016 Dec 5.

Abstract

Background/aims: To study the spectrum-effect relationship and effective components of Ligusticum Chuanxiong Hort. (LCH) on the protection of canine myocardial ischemia.

Methods: Fingerprint spectrum of LCH extracts was developed using high performance liquid chromatography (HPLC), and a canine model of acute myocardial ischemia was established by ligating the coronary artery. Bivariate correlation analysis and multivariate regression analysis were used to correlate the pharmacodynamics of LCH extract and its common peaks in HPLC.

Results: The bioactive components of LCH were ligustrazine, ferulic acid, cnidilide and ligustilide. Ligustrazine and ferulic acid could significantly reduce serum lactic acid in canine model of acute myocardial ischemia, while ligustilide could significantly reduce the elevation of serum free fatty acid.

Conclusions: The spectrum-effect relationship study shows that the effective components of LCH are ligustrazine, ferulic acid, cnidilide and ligustilide, which have protective effect on myocardial ischemia.

MeSH terms

  • 4-Butyrolactone / analogs & derivatives
  • 4-Butyrolactone / pharmacology
  • 4-Butyrolactone / therapeutic use
  • Animals
  • Cardiotonic Agents / analysis*
  • Cardiotonic Agents / pharmacology
  • Cardiotonic Agents / therapeutic use*
  • Chromatography, High Pressure Liquid
  • Coumaric Acids / pharmacology
  • Coumaric Acids / therapeutic use
  • Disease Models, Animal
  • Dogs
  • Ligusticum / chemistry*
  • Myocardial Infarction / blood
  • Myocardial Infarction / complications
  • Myocardial Infarction / drug therapy
  • Myocardial Infarction / pathology
  • Myocardial Ischemia / blood
  • Myocardial Ischemia / complications
  • Myocardial Ischemia / drug therapy*
  • Myocardial Ischemia / pathology
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Reference Standards
  • Rhizome / chemistry

Substances

  • Cardiotonic Agents
  • Coumaric Acids
  • Plant Extracts
  • ligustilide
  • ferulic acid
  • 4-Butyrolactone