[First attempts in the introduction of cryopreservation of ovarian tissues]

Orv Hetil. 2016 Dec;157(49):1947-1954. doi: 10.1556/650.2016.30582.
[Article in Hungarian]

Abstract

Introduction: The oncological treatment may damage ovarian function. To prevent this, it is possible to cryopreserve the ovarian tissue, and to keep the samples for long-term storage. The frozen-thawed tissue could be retransplanted after chemo- or radiotherapy.

Aim: The aim of our study was to examine the effect of cryopreservation on the viability of ovarian tissue.

Method: We analyzed the survival of frozen-thawed donated ovarian tissues. The quality of the follicles and hormone production in fresh and frozen-thawed samples were compared.

Results: Histological analysis showed that the number of viable follicles was reduced by 23% in the frozen-thawed samples. However, viable follicles still presented in post thawing ovarian tissues. Maximal estradiol production in frozen-thawed tissues was 908 pg/ml and hormone production was similar to the control tissues. The maximal progesterone production was 1.95 ng/ml post thawing, but these values were lower than the progesterone production of fresh tissues.

Conclusions: The method of ovarian cryopreservation used in our laboratory was able preserve the viability of follicles in frozen-thawed ovarian tissues. Orv. Hetil., 2016, 157(49), 1947-1954.

Keywords: chemotherapy; infertility; kemoterápia; meddőség; ovarian tissue cryopreservation; ovarian tissue transplantation; petefészek-transzplantáció; petefészekszövet-fagyasztás.

MeSH terms

  • Cryopreservation / methods*
  • Female
  • Humans
  • Immunohistochemistry
  • Oocytes / cytology*
  • Ovarian Follicle / cytology*
  • Ovary / cytology*
  • Ovary / immunology
  • Tissue Preservation
  • Tissue Survival*